【大师讲坛】第248期:单分子的力量:从闪烁的单分子到(生物)材料研究与应用
单分子光谱学在其应用的每一个领域都产生了巨大的影响,范围从催化、等离激元学、聚合物物理、生物物理到细胞生物学和DNA测序。此外,单分子技术使研究人员能够将荧光显微镜的分辨率推至衍射极限以下。在本次报告中,我将概述近年来(基于单分子的)显微镜模态的发展,这些发展与专用标记物和连接化学的合成密切相关。我将通过展示我实验室中当前的主要研究方向,来说明这些显微镜技术的发展如何推动了生物/材料科学的研究。
Single molecule spectroscopy has tremendously impacted every field in which the technique was applied, ranging from catalysis over plasmonics, polymer physics, biophysics to cell biology and DNA sequencing. Furthermore, single molecule techniques have allowed researchers to push the resolution of fluorescence microscopy past the diffraction limit. In this presentation, I will give an overview of recent (single molecule based) microscopy modality developments that go hand in hand with the synthesis of dedicated labels and linker chemistry. I will show how these microscopy developments have been driving research in bio/material science, by exemplifying major current research lines in my laboratory.